gugladv.blogg.se - Define precipitate

The process of precipitation has some requirements involving the valency of both the antigen and the antibody.The interaction between the soluble antigen and antibody results in the formation of insoluble lattice that forms precipitate out of the solution.Precipitation is a process where soluble antigens bind with their specific antibody at an optimum temperature and pH, resulting in the formation of an insoluble precipitate. The agglutination formed is then used to quantify the DNA strands by q-PCR.In this technique, the antibodies bind and agglutinate with antigen-DNA conjugates which enables the DNA strands to ligate with the antibodies.

A modified and more sensitive technique associated with agglutination is agglutination-PCR.

Agglutination has been used for the detection of antigens in bacteria which ultimately helps in the identification of those bacteria.

Because the process is quick and straightforward, these diagnostic tests are preferred over other sophisticated tests.

Different forms of agglutination are used in diagnostic tests which include latex agglutination, flocculation tests, direct bacterial agglutination, and hemagglutination.

The sensitivity and accuracy of the result depend on a number of factors like the duration of incubation with the antibody, amount and strength of the antigen conjugated to the carrier, and test conditions like the pH and protein concentration.

The result of this test can be collected based on the observation of clumps resulting from that antigen-antibody complex formation.

These conjugated particles then react with the patient’s serum which might or might not contain antibodies.

The agglutination process involving red blood cells is termed hemagglutination, and the process with white blood cells is termed leukoagglutination.The carrier can either be artificial (such as latex or charcoal particles) or biological (such as red blood cells).Agglutination reactions are used to particulate test antigens that are usually conjugated to a carrier.Agglutination is the process of clumping of antigens with their respective antibodies.